Physical-chemical, microbiological and functional characterization of the extracts of the species purple basil (Ocimum Sanctum)

Investigation article

Authors

  • Saltos-Bravo Mercedes Universidad Técnica de Manabí, UTM. Portoviejo, Ecuador.
  • Vélez-Álava Paola Universidad Técnica de Manabí, UTM. Portoviejo, Ecuador.

Keywords:

purple basil, antioxidant, essential oils, extraction, extracts

Abstract

The Purple Basil (Ocimum sanctum) is described as a medicinal and aromatic plant that has antispasmodic, antibacterial, anti-inflammatory, stimulant, sedative, among others properties. For this reason, the purpose of this research consisted of a physical, chemical, microbiological and functional characterization of the extracts obtained by the different extraction methods such as Clevenger (Hydrodistillation), Soxhlet, ultrasound, maceration, leaching, extracts that were evaluated for possible pharmaceutics use. Through this work we managed to determine physical and chemical properties (yield, organoleptic properties, density, pH). Likewise, the antimicrobial activity was evaluated by the antibiogram technique of diffusion of disc-plate in agar, tested in gram-negative bacteria like Salmonella and E. coli, in concentrations of 0, 25, 50, 75, 100 (mg/mL) respectively, showing that these Microorganisms are more sensitive to essential oil at a concentration of 60,27 (mg/mL). Finally, the essential oil was the one that showed better results in all the analyses carried out so that it is emphasized in the use of this for the future development of phytopharmaceuticals.

Keywords: purple basil, antioxidant, essential oils, extraction, extracts.

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References

Damian P. & Damian K. (1995). Aromatheray: scent and psyque, Healing Arts Press, Vermont. USA. Connor, A. J. (2002). Changes in fruit antioxidant activity storage. J. Agric. Food Chem. 50, 893-898.

Deás M, S. A. (2005). estudio del efecto hipoglicemiante del (Ocimun Sanctun L.) con el uso de un ensayo biológico en ratones. Recuperado el 1997, de http://www.bvs.sld.cu/revistas/pla/vol2_1_97/pla04197.htm

Fonnegra, G. R. (2007). Plantas medicinales aprobadas en Colombia. Segunda Edición.

Jayasinghe, C. N. (2003). Phenolics Composition and Antioxidant Activity of Sweet Basil (Ocimum basilicum L.) Agric. Food Chem. 51, 4442-4449

Jayasinghe, C. N. (2003). Phenolics Composition and Antioxidant Activity of Sweet Basil (Ocimum basilicum L.) Agric. Food Chem, 51, 4442-4449.

Lizcano, A. J. y Vergara, J. L. 2008. Evaluación de la actividad antimicrobiana de los extractos etanólicos y/o aceites esenciales de las especies vegetales Valeriana pilosa, Hesperomeles ferruginea, Myrcianthes rhopaloides y Passiflora manicata frente a microorganismos patógenos y fitopatogenos 88 Beuchat LR. 2001. Control of foodborne pathogens and spoilage microorganisms by naturally occurring antimicrobials. En: Microbial Food Contamination. Wilson CL, S Droby. (Ed.). CRC Press. London, UK. pp: 149-169.

Shahidi, F. P. (1992). Phenolic Antioxidants. Crit. Rev. Food Sci. Nutr. 32, 67-103.

Vanaclocha, B., & Cañigueral, S. (2003). Fitoterapia Vademécum de prescripción. Elsevier, Barcelona, España. Laboratorio de Remedios Herbolarios

Wang, H. G. (1996). Total antioxidant capacity of fruits. J. Agric. Food Chem. 44, 701-705.

Zheng, W. (2001). Antioxidant activity and phenolic compounds in selected herbs. J. Agric. Food Chem. 49, 5165-5170.

Published

2019-07-10

How to Cite

Saltos-Bravo, M., & Vélez-Álava, P. (2019). Physical-chemical, microbiological and functional characterization of the extracts of the species purple basil (Ocimum Sanctum): Investigation article. Scientific Journal INGENIAR: Engineering, Technology and Research, 2(4), 2-11. Retrieved from https://www.journalingeniar.org/index.php/ingeniar/article/view/13